Manual annotation is seldom the most adequate alternative: depending on the size of the target data, it can take an important amount of time and energy, is not objective, and is not reproducible. You might want to use manual editing to correct mistakes of automated segmenting or tracking, to do a full manual annotation of a dataset, or to create a “ground-truth” data. This tutorial show how to manually edit, correct and create spots and tracks in TrackMate.
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